ORFeome Collaboration: Introduction

DNA spotting robot

The ORFeome Collaboration (OC) was formed, in late 2005, to meet the need of the research community for an unrestricted source of fully sequence-validated full-ORF human cDNA clones in a format allowing easy transfer of the ORF sequences into virtually any type of expression vector. A major goal is to provide at least one fully sequenced full-ORF clone for each of the ~18,500 currently defined human genes. These clones are to be made available, without restriction, to researchers worldwide through multiple commercial distributors.

The Gateway™ cloning system (1, 2) was adopted by the OC for use with all of its clones. This site-specific recombinational cloning system allows for efficient transfer of ORF sequence from one vector (Entry vector) to any other expression vector modified with the requisite recombination sites flanking the insertion site for the ORF (Destination vector). ORFs transferred in this way have been found to acquire sequence changes only very rarely; thus for most purposes transferred sequences require no additional sequence analysis.

The OC has focused primarily, to date, on providing Gateway full-ORF clones for human genes. Approximately 2000 Gateway full-ORF mouse clones, however, are also listed in the OC collection. These mouse clones were contributed by the MGC, as a result of its effort to synthesize full-ORF Gateway clones for additional human and mouse genes.