The ORFeome Collaboration

Information for 1 library


   1. Barstead MPL-RB9

Barstead MPL-RB9

Name: Barstead MPL-RB9
Library ID: 564
Organism: Mus musculus
Strain: FVB/N
Age: 0
Stage: adult
Organ: bowel
Tissue: small bowel harvested 72 hours after irradiation with 1400 Gys
Host: DH10B
Vector: pT7T3D-PacI
Vector type: plasmid
Insert digest: 5' EcoRI/NotI 3'
Stop Codon Status: without
Description: 1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGTCGACATC], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation). Source irradiated bowel harvested 72 hours after irradiation (1400 Gys).